NBP2-42614

antibody from Novus Biologicals

Targeting: ACAT2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-42614

Provider

Novus Biologicals

Product name

Mouse Monoclonal ACAT2 Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ul

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HepG2 cell lysate Lane 2: Ramos cell lysate Lane 3: MCF-7 cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: SW480 cell lysate

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human ACAT2 protein