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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- GTX84970 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84970, RRID:AB_10728846
- Product name
- ACAT2 antibody [5C9]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2 .
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-ACAT2 mouse monoclonal antibody (GTX84970) immunofluorescent staining of COS7 cells transiently transfected with ACAT2
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody (GTX84970).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACAT2 mouse monoclonal antibody. (GTX84970, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (GTX84970, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACAT2 mouse monoclonal antibody. (GTX84970, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody. (GTX84970, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (GTX84970, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody(GTX84970), and then analyzed by flow cytometry.
