H00000039-M01

antibody from Abnova Corporation

Targeting: ACAT2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000039-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000039-M01, RRID:AB_1136883

Product name

ACAT2 monoclonal antibody (M01), clone 4A5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant ACAT2.

Antigen sequence

MNAGSDPVVIVSAARTIIGSFNGALAAVPVQDLGS
TVIKEVLKRATVAPEDVSEVIFGHVLAAGCGQNPV
RQASVGAGIPYSVPAWSCQMICGSGLKAVCLAVQS
IGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEM
PLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSR
EDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTR
RGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGT
VTPANASGINDGAAAVVLMKKSEADKRGLTPLARI
VSWSQVGVEPSIMGIGPIPAIKQAVTKAGWSLEDV
DIFEINEAFAAVSAAIVKELGLNPEKVNIEGGAIA
LGHPLGASGCRILVTLLHTLERMGRSRGVAALCIG
GGMGIAMCVQRE


Isotype

IgG

Antibody clone number

4A5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ACAT2 expression in transfected 293T cell line by ACAT2 monoclonal antibody (M01), clone 4A5.Lane 1: ACAT2 transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ACAT2 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol