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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [3]
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Validation data
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- Product number
- TA501216 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501216, RRID:AB_11125610
- Product name
- ACAT2 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)
- Antibody type
- Monoclonal
- Description
- ACAT2 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ACAT2
- Isotype
- IgG
- Antibody clone number
- OTI1C5
- Vial size
- 100 µl
- Concentration
- 0.84 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ACAT2 mouse monoclonal antibody (TA501216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2(RC201821).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-ACAT2 mouse monoclonal antibody (TA501216).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody(TA501216), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ACAT2 antibody(TA501216),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-ACAT2 antibody(TA501216),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
