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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- RQ7052 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ACAT2 Antibody / Acetyl-CoA acetyltransferase, cytosolic
- Antibody type
- Polyclonal
- Description
- This highly specific ACAT2 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ACAT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human HeLa, 3) human Jurkat, 4) human K562, 5) rat liver, 6) rat RH35 and 7) mouse liver tissue lysate with ACAT2 antibody. Predicted molecular weight: ~41 kDa (isoform 1) and ~45 kDa (isoform 2).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human HeLa, 3) human Jurkat, 4) human K562 and 5) mouse liver tissue lysate with ACAT2 antibody. Predicted molecular weight: ~41 kDa (isoform 1) and ~45 kDa (isoform 2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HepG2 cells with ACAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACAT2 antibody.
