Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX22797 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22797, RRID:AB_369085
- Product name
- TCP1 alpha antibody [91A]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Simian, Yeast
- Host
- Rat
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunofluorescent analysis of TCP1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with TCP1 antibody [91A] at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.