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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- NBP2-59347 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TCP1 alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Drosophila, Guinea Pig, Hamster, Porcine, Rabbit, Simian, Yeast
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at -20C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCP1 alpha Antibody (91a) [NBP2-59347] - Analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody (91a) [NBP2-59347] - Analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. Heat Shocked at 42 degrees C for 1h.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody (91a) [NBP2-59347] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. Heat Shocked at 42 degrees C for 1h.
