Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M02389 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TCP1 alpha Antibody Picoband™ (monoclonal, 2E7)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for TCP1 alpha detection. Tested with WB, IHC-P, ICC/IF, FCM in Human.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2.00E+07
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.
Ma X, Lu C, Chen Y, Li S, Ma N, Tao X, Li Y, Wang J, Zhou M, Yan YB, Li P, Heydari K, Deng H, Zhang M, Yi C, Ge L
Cell 2022 Apr 14;185(8):1325-1345.e22
Cell 2022 Apr 14;185(8):1325-1345.e22
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of TCP1 alpha using anti-TCP1 alpha antibody (M02389). TCP1 alpha was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-TCP1 alpha Antibody (M02389) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
- Additional image