MA3-026

antibody from Invitrogen Antibodies

Targeting: TCP1 CCT1, Ccta, D6S230E

Western blot

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

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Validation data

Product number: MA3-026 - Provider product page
Provider: Invitrogen Antibodies
Product name: TCP1 Monoclonal Antibody (91A)
Antibody type: Monoclonal
Antigen: Other
Description: MA3-026 detects T Complex Polypeptide 1 (TCP-1) from human, canine, chicken, hamster, mouse, primate, rat and yeast samples. MA3-026 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this clone detects a ~57 kDa band representing TCP-1 in mouse 3T3 cells. Immunofluorescence staining of TCP-1 in mouse germ line cells with MA3-026 results in diffuse cytoplasmic staining. The MA3-026 immunizing peptide corresponds to amino acid residues 306-556 from mouse TCP-1. The epitope for this antibody has been mapped to amino acids 465-469 representing the pentamer peptide AKLRA.
Reactivity: Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Yeast
Host: Rat
Isotype: IgG
Antibody clone number: 91A
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: -20° C, Avoid Freeze/Thaw Cycles

TCP1 protein structure - MA3-026 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TCP1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TCP1 Monclonal Antibody (91A) (Product # MA3-026) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TCP1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TCP1 Monclonal Antibody (91A) (Product # MA3-026) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TCP1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TCP1 Monclonal Antibody (91A) (Product # MA3-026) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.