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- Product number
- A03360 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ALKBH5 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for ALKBH5 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Role of RNA m(6)A modification in titanium dioxide nanoparticle-induced acute pulmonary injury: An in vitro and in vivo study.
The RNA demethylase ALKBH5 promotes the progression and angiogenesis of lung cancer by regulating the stability of the LncRNA PVT1.
RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological Assessment.
Ruan F, Liu C, Wang Y, Cao X, Tang Z, Xu J, Zeng J, Yin H, Zheng N, Yang C, Zuo Z, He C
Environmental pollution (Barking, Essex : 1987) 2022 Oct 15;311:119986
Environmental pollution (Barking, Essex : 1987) 2022 Oct 15;311:119986
The RNA demethylase ALKBH5 promotes the progression and angiogenesis of lung cancer by regulating the stability of the LncRNA PVT1.
Shen W, Pu J, Zuo Z, Gu S, Sun J, Tan B, Wang L, Cheng J, Zuo Y
Cancer cell international 2022 Nov 15;22(1):353
Cancer cell international 2022 Nov 15;22(1):353
RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological Assessment.
Ruan F, Zeng J, Yin H, Jiang S, Cao X, Zheng N, Han C, Zhang C, Zuo Z, He C
Small methods 2021 Mar;5(3):e2001045
Small methods 2021 Mar;5(3):e2001045
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ALKBH5 using anti-ALKBH5 antibody (A03360). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HepG2 whole cell lysates. Lane 4: human U20S whole cell lysates. Lane 5: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH5 antigen affinity purified polyclonal antibody (Catalog # A03360) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALKBH5 at approximately 52KD. The expected band size for ALKBH5 is at 52KD.
- Additional image
