HPA007196

antibody from Atlas Antibodies

Targeting: ALKBH5 FLJ20308, OFOXD1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA007196

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA007196, RRID:AB_1850461

Product name

Anti-ALKBH5

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ALKBH5, Gene description: AlkB family member 5, RNA demethylase, Alternative Gene Names: FLJ20308, OFOXD1, Validated applications: IHC, WB, Uniprot ID: Q6P6C2, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.3 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

m6A modification of mutant huntingtin RNA promotes the biogenesis of pathogenic huntingtin transcripts

Pupak A, Rodríguez-Navarro I, Sathasivam K, Singh A, Essmann A, del Toro D, Ginés S, Mouro Pinto R, Bates G, Vang Ørom U, Martí E, Brito V
EMBO Reports 2024;25(11):5026-5052

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Epitranscriptomic m 6 A modifications during reactivation of HIV-1 latency in CD4 + T cells

Mishra T, Phillips S, Zhao Y, Wilms B, He C, Wu L, Engelman A
mBio 2024;15(11)

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Astrocytic ALKBH5 in stress response contributes to depressive-like behaviors in mice

Guo F, Fan J, Liu J, Kong P, Ren J, Mo J, Lu C, Zhong Q, Chen L, Jiang H, Zhang C, Wen Y, Gu T, Li S, Fang Y, Pan B, Gao T, Cao X
Nature Communications 2024;15(1)

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Deubiquitinase USP9X stabilizes RNA m6A demethylase ALKBH5 and promotes acute myeloid leukemia cell survival

Wang P, Wang J, Yao S, Cui M, Cheng Y, Liu W, Gao Z, Hu J, Zhang J, Zhang H
Journal of Biological Chemistry 2023;299(8):105055

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The RNA m6A demethylase ALKBH5 drives emergency granulopoiesis and neutrophil mobilization by upregulating G-CSFR expression

Liu Y, Song R, Lu Z, Zhao L, Zhan X, Li Y, Cao X
Cellular & Molecular Immunology 2023;21(1):6-18

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N 6 -Methyladenosine and Reader Protein YTHDF2 Enhance the Innate Immune Response by Mediating DUSP1 mRNA Degradation and Activating Mitogen-Activated Protein Kinases during Bacterial and Viral Infections

Feng J, Meng W, Chen L, Zhang X, Markazi A, Yuan W, Huang Y, Gao S, Meng X
mBio 2023;14(1)

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M6A RNA Methylation Regulates Histone Ubiquitination to Support Cancer Growth and Progression

Yadav P, Subbarayalu P, Medina D, Nirzhor S, Timilsina S, Rajamanickam S, Eedunuri V, Gupta Y, Zheng S, Abdelfattah N, Huang Y, Vadlamudi R, Hromas R, Meltzer P, Houghton P, Chen Y, Rao M
Cancer Research 2022;82(10):1872-1889

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m6A demethylase ALKBH5 is required for antibacterial innate defense by intrinsic motivation of neutrophil migration

Liu Y, Song R, Zhao L, Lu Z, Li Y, Zhan X, Lu F, Yang J, Niu Y, Cao X
Signal Transduction and Targeted Therapy 2022;7(1)

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Protocol for the generation of HIV-1 genomic RNA with altered levels of N6-methyladenosine

Phillips S, Baek A, Kim S, Chen S, Wu L
STAR Protocols 2022;3(3):101616

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Global profiling reveals common and distinct N6-methyladenosine (m6A) regulation of innate immune responses during bacterial and viral infections

Feng J, Zhang T, Sorel O, Meng W, Zhang X, Lai Z, Yuan W, Chen Y, Huang Y, Gao S
Cell Death & Disease 2022;13(3)

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Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection

Wang L, Wu J, Liu R, Chen W, Pang Z, Zhou F, Xia L, Huang J, Pan T, Su X, Wang X
Frontiers in Immunology 2022;13

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Comprehensive Analysis of the Transcriptome-Wide m6A Methylation in Mouse Pachytene Spermatocytes and Round Spermatids

Hong S, Shen X, Cheng J, Tang H, Sun F
Frontiers in Genetics 2022;13

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Comparative analysis of the testes from wild-type and Alkbh5-knockout mice using single-cell RNA sequencing

Hong S, Shen X, Luo C, Sun F, Ma J
G3 Genes|Genomes|Genetics 2022;12(8)

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m6A modifications regulate intestinal immunity and rotavirus infection

Wang A, Tao W, Tong J, Gao J, Wang J, Hou G, Qian C, Zhang G, Li R, Wang D, Ren X, Zhang K, Ding S, Flavell R, Li H, Pan W, Zhu S
eLife 2022;11

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m(6)A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer.

Tsuchiya K, Yoshimura K, Iwashita Y, Inoue Y, Ohta T, Watanabe H, Yamada H, Kawase A, Tanahashi M, Ogawa H, Funai K, Shinmura K, Suda T, Sugimura H
Cancer gene therapy 2022 Oct;29(10):1355-1372

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Regulation of telomere homeostasis and genomic stability in cancer by N 6 -adenosine methylation (m 6 A)

Lee J, Hong J, Zhang Z, de la Peña Avalos B, Proietti C, Deamicis A, Guzmán G. P, Lam H, Garcia J, Roudier M, Sisk A, De La Rosa R, Vu K, Yang M, Liao Y, Scheirer J, Pechacek D, Yadav P, Rao M, Zheng S, Johnson-Pais T, Leach R, Elizalde P, Dray E, Xu K
Science Advances 2021;7(31)

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m 6 A demethylase ALKBH5 controls CD4 + T cell pathogenicity and promotes autoimmunity

Zhou J, Zhang X, Hu J, Qu R, Yu Z, Xu H, Chen H, Yan L, Ding C, Zou Q, Ye Y, Wang Z, Flavell R, Li H
Science Advances 2021;7(25)

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Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response

Yu F, Wei J, Cui X, Yu C, Ni W, Bungert J, Wu L, He C, Qian Z
Nucleic Acids Research 2021;49(10):5779-5797

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MYC promotes cancer progression by modulating m6A modifications to suppress target gene translation

Wu G, Suo C, Yang Y, Shen S, Sun L, Li S, Zhou Y, Yang D, Wang Y, Cai Y, Wang N, Zhang H, Yang Y, Cao J, Gao P
EMBO reports 2021;22(3)

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N6-methyladenosine modification of HIV-1 RNA suppresses type-I interferon induction in differentiated monocytic cells and primary macrophages

Evans D, Chen S, Kumar S, Espada C, Tirumuru N, Cahill M, Hu L, He C, Wu L
PLOS Pathogens 2021;17(3):e1009421

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m6A demethylase ALKBH5 suppression contributes to esophageal squamous cell carcinoma progression

Xiao D, Fang T, Lei Y, Xiao S, Xia J, Lin T, Li Y, Zhai J, Li X, Huang S, Jia J, Tian Y, Lin X, Cai K, Sun Y
Aging 2021;13(17):21497-21512

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m(6)A modification regulates lung fibroblast-to-myofibroblast transition through modulating KCNH6 mRNA translation.

Zhang JX, Huang PJ, Wang DP, Yang WY, Lu J, Zhu Y, Meng XX, Wu X, Lin QH, Lv H, Xie H, Wang RL
Molecular therapy : the journal of the American Society of Gene Therapy 2021 Dec 1;29(12):3436-3448

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RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia

Shen C, Sheng Y, Zhu A, Robinson S, Jiang X, Dong L, Chen H, Su R, Yin Z, Li W, Deng X, Chen Y, Hu Y, Weng H, Huang H, Prince E, Cogle C, Sun M, Zhang B, Chen C, Marcucci G, He C, Qian Z, Chen J
Cell Stem Cell 2020;27(1):64-80.e9

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m6A Reader YTHDC2 Promotes Radiotherapy Resistance of Nasopharyngeal Carcinoma via Activating IGF1R/AKT/S6 Signaling Axis

He J, Li Z, Rong Z, Gao J, Mu Y, Guan Y, Ren X, Zi Y, Liu L, Fan Q, Zhou M, Duan Y, Zhou Q, Deng Y, Sun L
Frontiers in Oncology 2020;10

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HIV-1 envelope proteins up-regulate N6-methyladenosine levels of cellular RNA independently of viral replication

Tirumuru N, Wu L
Journal of Biological Chemistry 2019;294(9):3249-3260

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Atlas of quantitative single-base-resolution N6-methyl-adenine methylomes

Koh C, Goh Y, Goh W
Nature Communications 2019;10(1)

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m6A mRNA methylation regulates human β-cell biology in physiological states and in type 2 diabetes

De Jesus D, Zhang Z, Kahraman S, Brown N, Chen M, Hu J, Gupta M, He C, Kulkarni R
Nature Metabolism 2019;1(8):765-774

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Dynamic m6A modification regulates local translation of mRNA in axons

Yu J, Chen M, Huang H, Zhu J, Song H, Zhu J, Park J, Ji S
Nucleic Acids Research 2018;46(3):1412-1423

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Cross-talk among writers, readers, and erasers of m 6 A regulates cancer growth and progression

Panneerdoss S, Eedunuri V, Yadav P, Timilsina S, Rajamanickam S, Viswanadhapalli S, Abdelfattah N, Onyeagucha B, Cui X, Lai Z, Mohammad T, Gupta Y, Huang T, Huang Y, Chen Y, Rao M
Science Advances 2018;4(10)

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N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications

Wang Y, Li Y, Yue M, Wang J, Kumar S, Wechsler-Reya R, Zhang Z, Ogawa Y, Kellis M, Duester G, Zhao J
Nature Neuroscience 2018;21(2):195-206

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m 6 A Demethylase ALKBH5 Maintains Tumorigenicity of Glioblastoma Stem-like Cells by Sustaining FOXM1 Expression and Cell Proliferation Program

Zhang S, Zhao B, Zhou A, Lin K, Zheng S, Lu Z, Chen Y, Sulman E, Xie K, Bögler O, Majumder S, He C, Huang S
Cancer Cell 2017;31(4):591-606.e6

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Viral and cellular N6-methyladenosine and N6,2′-O-dimethyladenosine epitranscriptomes in the KSHV life cycle

Tan B, Liu H, Zhang S, da Silva S, Zhang L, Meng J, Cui X, Yuan H, Sorel O, Zhang S, Huang Y, Gao S
Nature Microbiology 2017;3(1):108-120

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Region-specific RNA m(6)A methylation represents a new layer of control in the gene regulatory network in the mouse brain.

Chang M, Lv H, Zhang W, Ma C, He X, Zhao S, Zhang ZW, Zeng YX, Song S, Niu Y, Tong WM
Open biology 2017 Sep;7(9)

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N6-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression

Tirumuru N, Zhao B, Lu W, Lu Z, He C, Wu L
eLife 2016;5