PA5-121176

antibody from Invitrogen Antibodies

Targeting: STXBP3 UNC-18C

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121176

Provider

Invitrogen Antibodies

Product name

STXBP3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: U-87MG, U-251MG, Jurkat, Mouse kidney, Mouse lung, Mouse spleen, Mouse brain, Rat brain. The target is usually found in the following locations: Cell membrane, Cytoplasm, cytosol. Immunogen sequence: KQVVHLNLAE DCMNKFKLNI EKLCKTEQDL ALGTDAEGQK VKDSMRVLLP VLLNKNHDNC DKIRAILLYI FSINGTTEEN LDRLIQNVKI ENESDMIRNW SYLGVPIVPQ SQQGKPLRKD RSAEETFQLS RWTPFIKDIM EDAIDNRLDS KEWPYCSQCP AVWNGSGAVS ARQKPRANYL EDRKNGSKLI VFVIGGITYS EVRCAYEVSQ AHKSCEVIIG STHVLTPKKL LDDIKMLNKP KDKVSLIKDE

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.01 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of STXBP3 in extracts of various cell lines. Samples were incubated with STXBP3 Polyclonal antibody (Product # PA5-121176) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of STXBP3 in U2OS cells using a STXBP3 Polyclonal antibody (Product # PA5-121176). Blue: DAPI for nuclear staining.