MA5-42589

antibody from Invitrogen Antibodies

Targeting: QKI QK3

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42589

Provider

Invitrogen Antibodies

Product name

QKI Recombinant Rabbit Monoclonal Antibody (1D1N6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: SKOV3, NIH/3T3, U-251MG, Neuro-2a, Mouse brain, Rat brain, Rat heart. The target is usually found in the following locations: Cytoplasm, Nucleus. Immunogen sequence: RTPTPAGPTI MPLIRQIQTA VMPNGTPHPT AAIVPPGPEA GLIYTPYEYP YTLAPATSIL EYPIEPSGVL GAVATKVRRH DMRVHPYQRI VTADRAATGN

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

1D1N6

Vial size

100 µL

Concentration

0.8 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of QKI in NIH/3T3 cells using a QKI Recombinant Monoclonal antibody (Product # MA5-42589). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of QKI in NIH/3T3 cells using a QKI Recombinant Monoclonal antibody (Product # MA5-42589). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of QKI in NIH/3T3 cells. Samples were incubated with QKI Monoclonal antibody (Product # MA5-42589) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of QKI in 300 μg extracts of NIH/3T3 cells. Samples were precipitated with 3 μg QKI Monoclonal antibody (Product # MA5-42589). Western blot was performed from the immunoprecipitate using QKI Monoclonal antibody (Product # MA5-42589) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of QKI in paraffin-embedded human colon carcinoma. Samples were incubated with QKI Monoclonal antibody (Product # MA5-42589) using a dilution of 1:1,000 (40x lens). Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of QKI in paraffin-embedded human placenta. Samples were incubated with QKI Monoclonal antibody (Product # MA5-42589) using a dilution of 1:1,000 (40x lens). Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of QKI in paraffin-embedded mouse colon. Samples were incubated with QKI Monoclonal antibody (Product # MA5-42589) using a dilution of 1:1,000 (40x lens). Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of QKI in paraffin-embedded rat colon. Samples were incubated with QKI Monoclonal antibody (Product # MA5-42589) using a dilution of 1:1,000 (40x lens). Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.