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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-30563 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- QKI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, rat brain.
- Concentration
- 0.99 mg/mL
Submitted references Quantitative proteomics identifies altered O-GlcNAcylation of structural, synaptic and memory-associated proteins in Alzheimer's disease.
Wang S, Yang F, Petyuk VA, Shukla AK, Monroe ME, Gritsenko MA, Rodland KD, Smith RD, Qian WJ, Gong CX, Liu T
The Journal of pathology 2017 Sep;243(1):78-88
The Journal of pathology 2017 Sep;243(1):78-88
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of QKI was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a QKI Polyclonal Antibody (Product # PA5-30563) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using QKI Polyclonal Antibody (Product # PA5-30563). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. QKI Polyclonal Antibody (Product # PA5-30563) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of QKI was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: QKI Polyclonal Antibody (Product # PA5-30563) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of QKI was performed in frozen sectioned E13.5 Rat brain tissue using QKI Polyclonal Antibody (Product # PA5-30563) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of QKI was performed in frozen-sectioned adult mouse cerebellum tissue using QKI Polyclonal Antibody (Product # PA5-30563) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using QKI (Product # PA5-30563) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Verification of O-GlcNAc quantification with western blotting analysis using lysate of the human brain tissue. (A) PEG labelling of O-GlcNAcylated proteins. First, the O-GlcNAc motif was labelled with GalNAz by GalT1 and UDP-GalNAz, and the N-glycans were removed by PNGase F. Second, the azide group was linked to a 5-kDa PEG tag through copper-free click chemistry. (B and C) The proteins were precipitated and separated by SDS-PAGE, and detected with antibody against SYNPO (B) or antibody against protein quaking (QKI) (C) through western blotting. beta-Tubulin was used as a loading control.
