PA1-1962

antibody from Invitrogen Antibodies

Targeting: PSMA5 ZETA

Provider product page for PA1-1962

Western blot

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [8]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [2]
Other assay [3]

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Product number: PA1-1962 - Provider product page
Provider: Invitrogen Antibodies
Product name: PSMA5 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-1962 detects proteasome 20S subunit alpha 5 from human, rat, mouse and hamster cell lysates.
Concentration: 1 mg/mL

PSMA5 protein structure - PA1-1962 shown in red.

Submitted references Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.

Markmiller S, Fulzele A, Higgins R, Leonard M, Yeo GW, Bennett EJ
Cell reports 2019 Apr 30;27(5):1356-1363.e3

Network Analysis of UBE3A/E6AP-Associated Proteins Provides Connections to Several Distinct Cellular Processes.

Martínez-Noël G, Luck K, Kühnle S, Desbuleux A, Szajner P, Galligan JT, Rodriguez D, Zheng L, Boyland K, Leclere F, Zhong Q, Hill DE, Vidal M, Howley PM
Journal of molecular biology 2018 Mar 30;430(7):1024-1050

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

Gerhardt C, Lier JM, Burmühl S, Struchtrup A, Deutschmann K, Vetter M, Leu T, Reeg S, Grune T, Rüther U
The Journal of cell biology 2015 Jul 6;210(1):115-33

ATM regulates proteasome-dependent subnuclear localization of TRF1, which is important for telomere maintenance.

McKerlie M, Lin S, Zhu XD
Nucleic acids research 2012 May;40(9):3975-89

Urocortin trafficking in cerebral microvessel endothelial cells.

Tu H, Kastin AJ, Bjorbaek C, Pan W
Journal of molecular neuroscience : MN 2007;31(2):171-81

Urocortin trafficking in cerebral microvessel endothelial cells.

Tu H, Kastin AJ, Bjorbaek C, Pan W
Journal of molecular neuroscience : MN 2007;31(2):171-81

Neuronal induction of the immunoproteasome in Huntington's disease.

Díaz-Hernández M, Hernández F, Martín-Aparicio E, Gómez-Ramos P, Morán MA, Castaño JG, Ferrer I, Avila J, Lucas JJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Dec 17;23(37):11653-61

Neuronal induction of the immunoproteasome in Huntington's disease.

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of HeLa cell extract using Product # PA1-1962. Lane 1 is PA1-1962. Lane 2 is PA1-1962 pre-incubated with peptide.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), Neuro-2a (Lane 3), Caki-1 (Lane 4), NTERA-2 (Lane 5), HCT 116 (Lane 6), Caco-2 (Lane 7), and tissue extract (30 µg lysate) of Mouse Liver (Lane 8). The blots were probed with Anti-PSMA5 Rabbit Polyclonal Antibody (Product # PA1-1962, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 28 kDa band corresponding to PSMA5 was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of HeLa cells using Product # PA1-1962.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Proteasome 20S Subunit alpha-5 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMA5 Rabbit Polyclonal (Product # PA1-1962) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6. Rpgrip1l deficiency results in an accumulation of proteasomal subunit components at the base of cilia. (A-D) Immunofluorescence on MEFs of E12.5 WT and Rpgrip1l -/- embryos (both genotypes: Psmd2, n = 7 embryos; Psmd3, n = 3 embryos; Psmd4, n = 3 embryos; Psma5, n = 5 embryos). At least 10 cilia per embryo were used for Psmd2, Psmd3, and Psmd4 quantification, respectively, and 20 cilia per embryo were used for Psma5 quantification. The ciliary axoneme is marked by acetylated alpha-tubulin (green). The BB is marked by Pcnt (green; white arrowheads; A) or gamma-tubulin (blue; B-D). Error bars show standard error of the mean. *, P < 0.05; ***, P < 0.001. Bars: (A, B, and D) 1 um; (C) 0.5 um.