PA1-16560

antibody from Invitrogen Antibodies

Targeting: P4HTM EGLN4, FLJ20262, HIFPH4, P4H-TM, PH-4, PH4, PHD4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Immunoelectron microscopy (Recommended by provider)

Antibody Data

Product number

PA1-16560

Provider

Invitrogen Antibodies

Product name

P4HTM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

This antibody does not react with mouse samples. Suggested positive control: antigen standard for PH (transient overexpression lysate), HeLa and human glioblastoma cell lysates.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of human PHD4, using Product # PA1-16560. Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P4HTM in 50 µg whole cell lysate from HeLa and 293T cells. Samples were incubated in P4HTM polyclonal antibody (Product # PA1-16560 using a dilution of 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P4HTM in 25 µg/lane whole cell lysate from human glioblastoma tumor cell lines or in vitro translated PHD4. Samples were incubated in P4HTM polyclonal antibody (Product # PA1-16560 using a dilution of 2 µg/mL. Detection: Chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P4HTM in whole cell lysate from HeLa cells, normoxic and hypoxic, respectively. Samples were incubated in P4HTM polyclonal antibody (Product # PA1-16560).