Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 526002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#526002, RRID:AB_2563444
- Product name
- Purified anti-human M-CSF
- Antibody type
- Monoclonal
- Antigen
- Human M-CSF
- Description
- M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells. It binds CD14+ monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, cytotoxicity for tumor cells as well as synthesis of inflammatory cytokines such as IL-1, TNF-α, and IFN-γ in monocytes. M-CSF induces RANKL production in mature human osteoclasts and, consequently, this makes M-CSF a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF production in human monocytes and human tumor cells. High levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- L1617F1
- Vial size
- 500 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of 50ng of recombinant human M-CSF(lane 1) and 50ng of recombinant mouse M-CSF (lane 2) using anti-human M-CSF antibody(clone L1617F1).
- Conjugate
- Unconjugated