AP6754c

antibody from Abcepta

Targeting: CSF1 M-CSF, MCSF, MGC31930

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6754c

Provider

Abcepta

Proper citation

Abgent Cat#AP6754c, RRID:AB_1967914

Product name

M-CSF Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody (Center)(Cat. #AP6754c). AP6754c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. "

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of M-CSF Antibody (Center)(Cat#AP6754c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

M-CSF Antibody (Center) (AP6754c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of M-CSF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

M-CSF Antibody (Center) (Cat. #AP6754c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50