Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF416 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse M-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human M-CSF is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q3U4F9
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Microglial pannexin-1 channel activation is a spinal determinant of joint pain.
Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
Mousseau M, Burma NE, Lee KY, Leduc-Pessah H, Kwok CHT, Reid AR, O'Brien M, Sagalajev B, Stratton JA, Patrick N, Stemkowski PL, Biernaskie J, Zamponi GW, Salo P, McDougall JJ, Prescott SA, Matyas JR, Trang T
Science advances 2018 Aug;4(8):eaas9846
Science advances 2018 Aug;4(8):eaas9846
Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T
Biochemical and biophysical research communications 2007 Aug 24;360(2):346-51
Biochemical and biophysical research communications 2007 Aug 24;360(2):346-51
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by M-CSF and Neutralization by Mouse M-CSF Antibody. Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse M-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF416). The ND50 is typically 0.015-0.035 µg/mL.