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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- ABIN2508736 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Colony Stimulating Factor 1 (Macrophage) (CSF1) antibody (Biotin)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF. Anti-Human M-CSF specific antibody was purified by affinity chromatography and then biotinylated.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 50 μg
- Storage
- -20°C
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western blot analysis of rat hippocampus and rat whole brain lysates: 1, 2. Anti-Neuropeptide Y2 Receptor antibody (ABIN2511169), (1:200). 3, 4. Anti-Neuropeptide Y2 Receptor antibody, preincubated with the control peptide antigen. Expression of Neuropeptide Y2 Receptor in rat cerebellum Immunohistochemical staining of frozen section of rat cerebellum using Anti-Neuropeptide Y2 Receptor antibody (ABIN2511169), (1:100). Neuropeptide Y2 Receptor (red) is detected in Purkinje cells. B. Nuclei were stained using DAPI as the counterstain. C. Merged image of A. and B. Expression of NPY2R in rat dorsal root ganglion cells Immunocytochemical staining of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-Neuropeptide Y2 Receptor antibody (ABIN2511169), (1:100) followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E. Magnification: A-C: x20 E-F: x100
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence
