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Western blot
Immunocytochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-23815 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- M-CSF Monoclonal Antibody (26730)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse M-CSF. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 26730
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Macrophage colony-stimulating factor 1 was performed using 70% confluent log phase SK-BR-3 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with M-CSF Monoclonal Antibody (26730) (Product # MA5-23815) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization in SK-BR-3 but not in Raji which is reported to be negative. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of M-CSF in M-NFS-60 mouse myelogenous leukemia lymphoblast cell line. Samples were incubated in M-CSF monoclonal antibody (Product # MA5-23815). Recombinant Human M-CSF stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human M-CSF (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human M-CSF Monoclonal Antibody. The ND50 is typically 0.005-0.020 µg/mL.
