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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-23948 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLL1 Monoclonal Antibody (251123)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human (rh) DLL3 or rhDLL4 is observed.
- Antibody clone number
- 251123
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DLL1 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in DLL1 monoclonal antibody (Product # MA5-23948) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DLL1 in immersion fixed paraffin-embedded sections of human breast cancer tissue. Samples were incubated with DLL1 monoclonal antibody (Product # MA5-23948) using a dilution of 1.7 µg/mL for 1 hour at room temperature followed by Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells.
