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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- PA5-42902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: GSRCALALAV LSALLCQVWS SGVFELKLQE FVNKKGLLGN RNCCRGGAGP
- Concentration
- 0.5 mg/mL
Submitted references Valproic acid stimulates myogenesis in pluripotent stem cell-derived mesodermal progenitors in a NOTCH-dependent manner.
Breuls N, Giarratana N, Yedigaryan L, Garrido GM, Carai P, Heymans S, Ranga A, Deroose C, Sampaolesi M
Cell death & disease 2021 Jul 5;12(7):677
Cell death & disease 2021 Jul 5;12(7):677
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Hela cell lysate using an anti-DLL1 polyclonal antibody (Product # PA5-42902). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HeLa cell lysate using an anti-DLL1 polyclonal antibody (Product # PA5-42902).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DLL1 Polyclonal Antibody (Product # PA5-42902) and ~78kDa band corresponding to DLL1 was observed across all the cell lines and tissues tested. Membrane extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), PANC-1 (Lane 3), MCF7 (Lane 4), THP-1 (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Rat Heart (Lane 8) and Mouse Heart (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 DLL1 overexpression improves the myogenic propensity of mesodermal progenitors. A Schematic overview of the doxycycline (dox)-inducible DLL1 construct flipped into an embryonic stem cell (ESC) master line. B Quantitative PCR for DLL1 after exposure of the generated ESC line to dox for two or four days. C Western blot for DLL1 and NOTCH1 after exposure of the generated ESC line to dox for four days. Tubulin (TUB) was used as a housekeeping protein. Quantification graph is on the right. D Immunofluorescence analysis of lamin A/C-positive mesodermal progenitors, with or without dox, (green) in co-culture with C2C12 myoblasts ( n = 3). Scale bars: 100 um. * P < 0.05.
