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Western blot
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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-42902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: GSRCALALAV LSALLCQVWS SGVFELKLQE FVNKKGLLGN RNCCRGGAGP Sequence homology: Cow: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Valproic acid stimulates myogenesis in pluripotent stem cell-derived mesodermal progenitors in a NOTCH-dependent manner.
Breuls N, Giarratana N, Yedigaryan L, Garrido GM, Carai P, Heymans S, Ranga A, Deroose C, Sampaolesi M
Cell death & disease 2021 Jul 5;12(7):677
Cell death & disease 2021 Jul 5;12(7):677
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 DLL1 overexpression improves the myogenic propensity of mesodermal progenitors. A Schematic overview of the doxycycline (dox)-inducible DLL1 construct flipped into an embryonic stem cell (ESC) master line. B Quantitative PCR for DLL1 after exposure of the generated ESC line to dox for two or four days. C Western blot for DLL1 and NOTCH1 after exposure of the generated ESC line to dox for four days. Tubulin (TUB) was used as a housekeeping protein. Quantification graph is on the right. D Immunofluorescence analysis of lamin A/C-positive mesodermal progenitors, with or without dox, (green) in co-culture with C2C12 myoblasts ( n = 3). Scale bars: 100 um. * P < 0.05.
