PA5-80008

antibody from Invitrogen Antibodies

Targeting: SLC16A4 MCT4, MCT5

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80008

Provider

Invitrogen Antibodies

Product name

SLC16A4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: Rat Testis Tissue, JURKAT Cell, HELA Cell, MCF-7 Cell. IHC: Human Mammary Cancer Tissue, Human Placenta Cancer Tissue.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Cancer-derived exosomal TRIM59 regulates macrophage NLRP3 inflammasome activation to promote lung cancer progression.

Liang M, Chen X, Wang L, Qin L, Wang H, Sun Z, Zhao W, Geng B
Journal of experimental & clinical cancer research : CR 2020 Aug 31;39(1):176

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SLC16A4 on paraffin-embedded human mammary cancer tissue. Sample was incubated with SLC16A4 polyclonal antibody (Product# PA5-80008).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SLC16A4 on paraffin-embedded human placenta cancer tissue. Sample was incubated with SLC16A4 polyclonal antibody (Product# PA5-80008).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Loss of ABHD5 causes metabolic reprogramming in macrophages. a . THP-1 cells treated with shCtrl or shABHD5 and control were stained by oil red, representative photographs were taken, then analyzed lipid content by a quantitative method. Scale bars, 50 mum. b . THP-1 macrophages infected with lentiviral vectors expressing TRIM59 or GFP control and control stained by oil red, then analyzed lipid content by a quantitative method. Scale bars, 50 mum. c - d . THP-1 cells stably transfected with shCtrl or shABHD5 ( c ) or infected with TRIM59 lentiviral particles or GFP lentiviral control ( d ). Lactate secretion ability was measured. e - f . Protein level of MCT4 was analyzed by immunoblot. All statistic data in this figure represent mean +- SEM. The significance was determined by Student's t -test. ** p < 0.01