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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MA5-42698 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PROK1 Recombinant Rabbit Monoclonal Antibody (1B4G2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: 293T, HepG2, Mouse liver, Mouse kidney, Rat ovary, Rat kidney.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 1B4G2
- Vial size
- 100 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of PROK1 in various cell lines using a PROK1 Recombinant Monoclonal antibody (Product # MA5-42698) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.
