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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P188GBT-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PROK1 Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hEG-VEGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EG-VEGF (500-P188G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEG-VEGF. Western Blot: To detect Human EG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EG-VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P188GBT-1MG will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Vial size
- 2 x 500 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human EG-VEGF by Western Blot analysis PROK1 Polyclonal Antibody, Biotin (Product # 500-P188GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EG-VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human EG-VEGF by Western Blot analysis PROK1 Polyclonal Antibody, Biotin (Product # 500-P188GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EG-VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect hEG-VEGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of PROK1 Polyclonal Antibody, Biotin (Product # 500-P188GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech PROK1 Polyclonal Antibody (Product # 500-P188G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEG-VEGF.
- Conjugate
- Biotin
