LS-C828386

antibody from LSBio

Targeting: CD53 MOX44, TSPAN25

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C828386

Provider

LSBio

Product name

CD53 Antibody (clone 7A8D3) LS-C828386

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

7A8D3

Storage

Store at 4°C for short term storage. Aliquot and store at -20°C for long term storage.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using CD53 mAb against HEK293 (1) and CD53 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using CD53 mAb against human CD53 (AA: extra mix) recombinant protein. (Expected MW is 37 kDa)

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis using CD53 mouse mAb against Raji (1), Ramos (2), Jurkat (3), MOLT4 (4), K562 (5), HL-60 (6), THP-1 (7), and U937 (8) cell lysate.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)