Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- Ab007146 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD53 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human CD53 Antibody, Monoclonal (HI29), could be used for Flow and so on. Application Flow: < 0.5 _g/100w cells Protein Function May be involved in growth regulation in hematopoietic cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- HI29
- Vial size
- 100_g,10_g,1mg,50_g
- Concentration
- Lot by Lot
- Storage
- Shipped at 4¡C. Store at 4¡C long term (36 months). Upon delivery aliquot.
No comments: Submit comment
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD53 Mouse mAb (Ab007146) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD53 with CD53 Mouse mAb (Ab007146) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD53 Mouse mAb (Ab007146) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD53 (red) with CD53 Mouse mAb (Ab007146) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD53 Mouse mAb (Ab007146) - Flow Cytometry Flow Cytometry analysis of Raji cells labelling CD53 (red) with CD53 Mouse mAb (Ab007146) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD53 Mouse mAb (Ab007146) - Flow Cytometry Flow Cytometry analysis of Jurkat cells labelling CD53 (red) with CD53 Mouse mAb (Ab007146) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
