Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- Ab181125 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD53 Mouse mAb (Biotin)
- Antibody type
- Monoclonal
- Description
- Application:Flow: ²0.5_g/testFor flow cytometric staining, the suggested use of this reagent is ²0.5 _g per million cells in 100 µL staining volume or 100 µL of whole blood.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Antibody clone number
- HI29
- Vial size
- 100_g,10_g,25_g
- Concentration
- 0,5 mg/ml
- Storage
- The antibody solution should be stored undiluted between 2¡C and 8¡C, and protected from prolonged exposure to light. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD53 Mouse mAb (Biotin) (Ab181125) - Flow Cytometry Flow cytometry analysis of CD53 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD53 Mouse mAb (Biotin) (Ab181125)at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
