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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [11]
- Flow cytometry [2]
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- Product number
- TA504220 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504220, RRID:AB_11139907
- Product name
- C20orf3 mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)
- Antibody type
- Monoclonal
- Description
- C20orf3 mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- APMAP
- Isotype
- IgG
- Antibody clone number
- OTI4F6
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C20orf3 (RC202410, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C20orf3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C20orf3 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-C20orf3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-C20orf3 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504220)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-C20orf3 antibody(TA504220),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-C20orf3 antibody(TA504220),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
