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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-109257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KV4.3 (KCND3) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: MCF-7, mouse marrow tissue, LOVO, MG-63, human esophagus tissue, human placenta tissue, mouse cerebellum tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KV4.3 in MG-63 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KV4.3 polyclonal antibody (Product # PA5-109257), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KV4.3 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KV4.3 polyclonal antibody (Product # PA5-109257), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KV4.3 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KV4.3 polyclonal antibody (Product # PA5-109257), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of KV4.3 in MG-63 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with KV4.3 polyclonal antibody (Product # PA5-109257) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
