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- Validations
- Immunocytochemistry [1]
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- Product number
- 710888 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT2B Recombinant Polyclonal Antibody (17HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 17HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Noggin regulates foregut progenitor cell programming, and misexpression leads to esophageal atresia.
Pinzon-Guzman C, Sangadala S, Riera KM, Popova EY, Manning E, Huh WJ, Alexander MS, Shelton JS, Boden SD, Goldenring JR
The Journal of clinical investigation 2020 Aug 3;130(8):4396-4410
The Journal of clinical investigation 2020 Aug 3;130(8):4396-4410
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence was performed on methanol fixed MDA-MB-231 cells for detection of WNT2B/13 using Anti-WNT2B/13 Recombinant Rabbit Monoclonal Antibody (Product # 701856, 2 µg/mL), alpha-Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000), Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594conjugate (Product # A-11032, 1:400) respectively. Panel a) shows representative cells that were stained for detection and localization of WNT2B/13 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938,). Panel c) represents cytoskeletal alpha-tubulin staining (red). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of WNT2B/13. Panel e) represents control cells with no primary antibody to assess background.
