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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-42480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT2B Recombinant Rabbit Monoclonal Antibody (JB48-34)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SiHa, Hela, LOVO, SHSY-5Y, human kidney tissue, mouse kidney tissue, rat testis tissue. Subcellular Location: Secreted. Extracellular region.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB48-34
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Wnt2b in Hela cells (green) using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Wnt2b in LOVO cells (green) using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Wnt2b in SHSY-5Y cells (green) using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of WNT2B in LOVO cells using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480) at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
