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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab134748 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Wnt2b Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Wnt2b Antibody, Recombinant, could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 287-300- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,3 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Wnt2b Antibody (Ab134748) - Western Blot All lanes: Recombinant Wnt2b Antibody (Ab134748) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 45 kDa Exposure time: 1.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Wnt2b Antibody (Ab134748) - IF/ICC Immunocytochemistry analysis of Wnt2b (green) in HEK-293T cells using Recombinant Wnt2b Antibody (Ab134748) and cells were counterstained with DAPI (blue).
