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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- LS-C19004 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C19004, RRID:AB_2220937
- Product name
- ABCB6 Antibody LS-C19004
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Canine
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Submitted references Classification of subcellular location by comparative proteomic analysis of native and density-shifted lysosomes.
Della Valle MC, Sleat DE, Zheng H, Moore DF, Jadot M, Lobel P
Molecular & cellular proteomics : MCP 2011 Apr;10(4):M110.006403
Molecular & cellular proteomics : MCP 2011 Apr;10(4):M110.006403
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag-tagged human ABCB6 protein at 104 kD and a truncated form of the protein at 79 kD (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A Lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag antibody as a positive control. The membrane was probe.
