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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A500-009A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MOV10 Monoclonal Antibody (15C1B8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 15C1B8
- Vial size
- 100 µL
- Concentration
- 40 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse MOV10 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse monoclonal anti-MOV10 antibody [15C1B8] (Product # A500-009A lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MOV10 by WB of immunoprecipitates from Jurkat lysate. Antibodies: Mouse monoclonal anti-MOV10 [15C1B8] (A500-009A) and rabbit anti-MOV10 (A301-571A). Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse MOV10 by WB of Jurkat, HeLa, 293T, and NIH3T3 lysate. Antibody: Mouse monoclonal anti-MOV10 [15C1B8] (A500-009A). Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MOV10 in FFPE testicular seminoma by IHC. Antibody: Mouse monoclonal MOV10 [15C1B8] (A500-009A). Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MOV10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Mouse monoclonal anti-MOV10 antibody [15C1B8] (Product # A500-009A lot 3) used for IP at 20 µL per reaction. MOV10 was also immunoprecipitated by rabbit anti-MOV10 antibody (Product # A301-571A). For blotting immunoprecipitated MOV10 (Product # A500-009A) was used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 10 seconds.
