LS-B15864

antibody from LSBio

Targeting: CYB5B CYB5-M

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B15864

Provider

LSBio

Product name

IHC-plus™ CYB5B Antibody LS-B15864

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: CYB5B antibody at 0.8 ug/ml. Lane 1: HepG-2 whole cell lysate. Lane 2: Mouse liver tissue. Lane 3: Mouse heart tissue. Lane 4: HeLa whole cell lysate. Lane 5: U87 whole cell lysate. Lane 6: EC109 whole cell lysate. Lane 7: A549 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 16 kDa. Observed band size: 16 kDa.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: CYB5B antibody at 0.8µg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: Mouse heart tissue Lane 4: Hela whole cell lysate Lane 5: U87 whole cell lysate Lane 6: EC109 whole cell lysate Lane 7: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using antibody at dilution of 1:100.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using antibody at dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using antibody at dilution of 1:100.