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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-30649 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASIC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: SK-N-SH, U87-MG, MCF-7.
- Concentration
- 0.42 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ASIC4 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASIC4 Polyclonal Antibody (Product # PA5-30649) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ASIC4 Polyclonal Antibody (Product # PA5-30649). Sample (30 µg of whole cell lysate). Lane A: U87-MG. B: MCF-7. 7.5% SDS PAGE. ASIC4 Polyclonal Antibody (Product # PA5-30649) diluted at 1:1,000.
