Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AP53289PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53289PU-N, RRID:AB_11142763
- Product name
- anti PHTF1 / PHTF (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 231-260) of human PHTF1 / PHTF.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of PHTF1 (arrow) in Hela cell line lysates (35ug/lane) using PHTF1 / PHTF antibody Cat.-No. AP53289PU-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human testis tissue (Formalin-fixed, Paraffin-embedded) using PHTF1 / PHTF antibody Cat.-No. AP53289PU-N, followed by FITC-conjugated goat anti-rabbit lgG (whole molecule); FITC emits green fluorescence.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human testis tissue (Formalin-fixed, Paraffin-embedded) using PHTF1 / PHTF antibody Cat.-No. AP53289PU-N, followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram) using PHTF1 / PHTF antibody Cat.-No. AP53289PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.