PA5-81196

antibody from Invitrogen Antibodies

Targeting: HIPK1 KIAA0630, MGC26642, MGC33446, MGC33548, Myak, Nbak2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81196

Provider

Invitrogen Antibodies

Product name

HIPK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HIPK1 in Lane A: NCI-H1299 Whole Cell Lysate (30 µg). Samples were probed using a HIPK1 Polyclonal Antibody (Product # PA5-81196) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:131 kDa. Observed band size:125 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HIPK1 Polyclonal Antibody (Product # PA5-81196) at 1:500 dilution. Lane A: NCI-H1299 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 131 kDa. Observed band size: 125 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of HIPK1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with HIPK1 Polyclonal Antibody (Product # PA5-81196, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human HIPK1 in human prostate with HIPK1 Polyclonal Antibody (Product # PA5-81196, 1:100,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human HIPK1 in human rectum with HIPK1 Polyclonal Antibody (Product # PA5-81196, 1:100,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HIPK1 Immunoprecipitation using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate, Lane B: 0.5 mg A549 Whole Cell Lysate 1 µL with HIPK1 Polyclonal Antibody (Product # PA5-81196) and 15 µL of 50 % Protein G agarose. Primary antibody: HIPK1 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 133 kDa. Observed band size: 105 kDa.