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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-96480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CSDE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSAQRNHAIR IKKLPKGTVS FHSHSDHRFL GTVEKEATFS NPKTTSPNKG KEKEAEDGII AYDDCGVKLT IAFQAKDVEG STSPQIGDKV EFSISDKQRP GQQVATCVRL LGRNSNSKRL LGYVATLKDN FGFIETANHD KEIFFHYSEF SGDVDSLELG DMVEYSLSKG KGNKVSAEKV NKTHSVNGIT EEADPTIYSG KVIRPLRSVD PTQTEYQGMI EIVEEGDMKG EV; Positive Samples: U-87MG, LO2, HeLa, MCF7, Mouse brain; Cellular Location: Cytoplasm
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.74 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CSDE1 in extracts from normal (control) and CSDE1 knockout (KO) HeLa cells. Samples were incubated with CSDE1 Polyclonal antibody (Product # PA5-96480) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CSDE1 in L929 cells. Samples were incubated with CSDE1 Polyclonal antibody (Product # PA5-96480) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CSDE1 in 100 μg extracts of HeLa cells. Samples were precipitated with 3 μg CSDE1 Polyclonal antibody (Product # PA5-96480). Western blot was performed from the immunoprecipitate using CSDE1 Polyclonal antibody (Product # PA5-96480) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of CSDE1 was performed in 100 µg extracts of HeLa cells using CSDE1 Polyclonal Antibody (Product # PA5-96480). Western blot was performed from the immunoprecipitate using CSDE1 Polyclonal Antibody.
