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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- HPA024177 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA024177, RRID:AB_1848798
- Product name
- Anti-SIKE1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SIKE1, Gene description: suppressor of IKBKE 1, Alternative Gene Names: FLJ21168, SIKE, Validated applications: ICC, IHC, WB, Uniprot ID: Q9BRV8, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Suppressor of IKKepsilon forms direct interactions with cytoskeletal proteins, tubulin and α‐actinin, linking innate immunity to the cytoskeleton
Sonnenschein H, Lawrence K, Wittenberg K, Slykas F, Dohleman E, Knoublauch J, Fahey S, Marshall T, Marion J, Bell J
FEBS Open Bio 2018;8(7):1064-1082
FEBS Open Bio 2018;8(7):1064-1082
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and SIKE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410899).
- Sample type
- Human
- Protocol
- Protocol
