PAB21658

antibody from Abnova Corporation

Targeting: SIKE1 FLJ21168, SIKE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21658

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21658, RRID:AB_10962336

Product name

RP5-1000E10.4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RP5-1000E10.4.

Antigen sequence

ESLVDQSAALHRRVAAMREAGTALPDQYQEDASDM
KDMSKYKPHILLSQENTQIRDLQQENRELWISLEE
HQDALEL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with RP5-1000E10.4 polyclonal antibody (Cat # PAB21658) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with RP5-1000E10.4 polyclonal antibody (Cat # PAB21658) at 1-4 ug/mL dilution shows positivity in focal adhesions.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine with RP5-1000E10.4 polyclonal antibody (Cat # PAB21658) strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)