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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-55003 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIKE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ESLVDQSAAL HRRVAAMREA GTALPDQYQE DASDMKDMSK YKPHILLSQE NTQIRDLQQE NRELWISLEE HQDALEL Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIKE in human cell line MCF-7 using a SIKE Polyclonal Antibody (Product # PA5-55003).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIKE in control (vector only transfected HEK293T lysate) and SIKE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SIKE Polyclonal Antibody (Product # PA5-55003).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SIKE in human cell line U-251 MG shows positivity in focal adhesion sites. Samples were probed using a SIKE Polyclonal Antibody (Product # PA5-55003).
