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Learn500-P95A-100UG
antibody from Invitrogen Antibodies
Targeting: CCL20
CKb4, exodus-1, LARC, MIP-3a, SCYA20, ST38
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
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- Product number
- 500-P95A-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIP-3 alpha Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3alpha (CCL20). Anti-Human MIP-3alpha (CCL20)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3alpha (CCL20) matrix. Sandwich ELISA: To detect Human MIP-3alpha (CCL20) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3alpha (CCL20) (500-P95ABt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3alpha (CCL20). Western Blot: To detect HumanMIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Involvement of CCR6/CCL20/IL-17 axis in NSCLC disease progression.
Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
Kirshberg S, Izhar U, Amir G, Demma J, Vernea F, Beider K, Shlomai Z, Wald H, Zamir G, Shapira OM, Peled A, Wald O
PloS one 2011;6(9):e24856
PloS one 2011;6(9):e24856
Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
Kriehuber E, Breiteneder-Geleff S, Groeger M, Soleiman A, Schoppmann SF, Stingl G, Kerjaschki D, Maurer D
The Journal of experimental medicine 2001 Sep 17;194(6):797-808
The Journal of experimental medicine 2001 Sep 17;194(6):797-808
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human MIP-3alpha (CCL20) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of MIP-3 alpha Polyclonal Antibody (Product # 500-P95A-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech MIP-3 alpha Polyclonal Antibody, Biotin (Product # 500-P95ABT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3alpha (CCL20).
