MAB256-100

antibody from Novus Biologicals

Targeting: NGF NGFB

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB256-100

Provider

Novus Biologicals

Product name

Mouse Monoclonal beta-NGF Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human beta-NGF in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) beta-NGF and recombinant rat (rr) beta-NGF is observed and less than 5% cross-reactivity with recombinant human (rh) BDNF, rhNT-3, and rhNT-4 is observed. This antibody neutralizes the bioactivity of rh beta-NGF, rm beta-NGF, and rr beta-NGF.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by beta-NGF and Neutralization by Human beta-NGF Antibody. Recombinant Human beta-NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta-NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human beta-NGF Monoclonal Antibody (Catalog # MAB256). The ND₅₀ is typically 0.1-0.4 µg/mL.