MAB256-100

antibody from R&D Systems

Targeting: NGF NGFB

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB256-100

Provider

R&D Systems

Product name

Human beta-NGF Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human beta-NGF in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) beta-NGF and recombinant rat (rr) beta-NGF is observed and less than 5% cross-reactivity with recombinant human (rh) BDNF, rhNT-3, and rhNT-4 is observed. This antibody neutralizes the bioactivity of rh beta-NGF, rm beta-NGF, and rr beta-NGF.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

CAA36832


Isotype

IgG

Antibody clone number

25623

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

The Role of Nerve Growth Factor in Maintaining Proliferative Capacity, Colony-Forming Efficiency, and the Limbal Stem Cell Phenotype.

Kolli S, Bojic S, Ghareeb AE, Kurzawa-Akanbi M, Figueiredo FC, Lako M
Stem cells (Dayton, Ohio) 2019 Jan;37(1):139-149

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RIP2 Gates TRAF6 Interaction with Death Receptor p75^NTR to Regulate Cerebellar Granule Neuron Survival.

Kisiswa L, Fernández-Suárez D, Sergaki MC, Ibáñez CF
Cell reports 2018 Jul 24;24(4):1013-1024

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Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages.

Oddone F, Roberti G, Micera A, Busanello A, Bonini S, Quaranta L, Agnifili L, Manni G
PloS one 2017;12(1):e0168565

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Functional Characterization of Human ProNGF and NGF Mutants: Identification of NGF P61SR100E as a "Painless" Lead Investigational Candidate for Therapeutic Applications.

Malerba F, Paoletti F, Bruni Ercole B, Materazzi S, Nassini R, Coppi E, Patacchini R, Capsoni S, Lamba D, Cattaneo A
PloS one 2015;10(9):e0136425

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Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.

Samah B, Porcheray F, Gras G
Clinical and experimental immunology 2008 Mar;151(3):476-86

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Nerve growth factor induces increased airway inflammation via a neuropeptide-dependent mechanism in a transgenic animal model of allergic airway inflammation.

Quarcoo D, Schulte-Herbrüggen O, Lommatzsch M, Schierhorn K, Hoyle GW, Renz H, Braun A
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2004 Jul;34(7):1146-51

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Nerve growth factor overexpression and autocrine loop in breast cancer cells.

Dollé L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H
Oncogene 2003 Aug 28;22(36):5592-601

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by beta-NGF and Neutralization by Human beta-NGF Antibody. Recombinant Human beta-NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta-NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human beta-NGF Monoclonal Antibody (Catalog # MAB256). The ND₅₀ is typically 0.1-0.4 µg/mL.