Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB556 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat beta-NGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat beta-NGF in ELISAs.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P25427
- Isotype
- IgG
- Antibody clone number
- 884530
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Proliferation Induced by beta-NGF and Neutralization by Rat beta-NGF Antibody. Recombinant Rat beta-NGF(Catalog # 556-NG) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Proliferation elicited by Recombinant Rat beta-NGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat beta-NGF Monoclonal Antibody (Catalog # MAB556). The ND50 is typically 0.05-0.25 μg/ml.