GTX54873

antibody from GeneTex

Targeting: NGF NGFB

ELISA (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX54873

Provider

GeneTex

Product name

NGF/proNGF Neutralizing antibody [HAL06]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Indirect ELISA of adsorbed neurotrophic factors was performed. Neurotrophic factors mouse NGF 2.5S, mouse NGF 7S, hI2-NGF, proNGF, hBDNF, hNT-3 and hNT-4 were adsorbed to a microplate and analyzed using NGF/proNGF Neutralizing antibody, followed HRP-conjudated secondary antibody and chromogenic substrate.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Indirect ELISA of adsorbed proNGF and NGF was performed. Increasing amounts of proNGF (blue) and mouse NGF 2.5S (red) were adsorbed to a microplate and detected with NGF/proNGF Neutralizing antibody, followed by HRP-conjudated secondary antibody and chromogenic substrate.

Other assay

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Neutralization effect on NGF-mediated PC12 proliferation was analyzed in the absence of serum and in the presence of mouse NGF 2.5S (50 ng/ml) as indicated. Varying concentrations of NGF/proNGF Neutralizing antibody were added to the culture and cell viability was established after 24 h. Note that 600 ng/ml NGF/proNGF Neutralizing antibody was able to completely block the activity of 50 ng/ml mouse NGF.

Validation comment

Neut

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Neutralizing assay of NGF-induced PC12 cells was performed (A) in the absence of NGF, (B) in the presence of mouse NGF 2.5S (50 ng/ml) and (C) in the presence of mouse NGF 2.5S (50 ng/ml) with NGF/proNGF Neutralizing antibody (1000 ng/ml). The neurite outgrowth over a period of 6 days was visualized by Methylene Blue staining.

Validation comment

Neut

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Neutralizing assay of proNGF-induced PC12 cells was performed (A) in the absence of proNGF, (B) in the presence of proNGF (100 ng/ml) and (C) in the presence of proNGF (100 ng/ml) with NGF/proNGF Neutralizing antibody (500 ng/ml). The neurite outgrowth over a period of 6 days was visualized by Methylene Blue staining.

Validation comment

Neut