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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-29425 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NGF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: NT2D1.
- Concentration
- 0.91 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NGF Polyclonal Antibody (Product # PA5-29425). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 12% SDS PAGE. NGF Polyclonal Antibody (Product # PA5-29425) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-NGF Polyclonal Antibody (Product # PA5-29425) and a 30 kDa band corresponding to pro-NGF was observed across in Rat Salivary Gland and not in Rat Brain and Mouse Salivary Gland. Whole cell extracts (30 µg lysate) of Mouse Salivary Gland (Lane 1), Rat Salivary Gland (Lane 2) and Rat Brain (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
